rat os umr106 cell line  (iCell Bioscience Inc)

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: Schematic diagram of anti-OS effect of HANPs associated with the morphology of particles and OS cell source. Among the six HANPs with different morphologies and particle sizes, the rod-like HANPs inhibit the growth of 143B cells most efficiently in vitro and in vivo, while needle-like HANPs are strongest to suppress the proliferation of UMR106 cells. The anti-OS capacity of HANPs correlates with the morphology of particles, endocytosis efficiency, the levels of intracellular Ca 2+ , oxidative damage, immunogenic cell death (ICD) and mitochondrial apoptosis, as well as the cell source.

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: In Vitro, In Vivo

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: Among the six HANPs, R-HANPs had the strongest inhibitory effect on the viability of 143B cells, and N-HANPs had the strongest inhibitory effect on the viability of UMR106 cells. The viabilities of 143B ( A ) and UMR106 ( B ) cells after the cells were treated with six HANPs at the different concentration of 100, 200, 400 and 800 μg/mL for 1, 2 and 3 days (n=3; vs 0 μg/mL, *P<0.05, **P<0.01; vs 400 μg/mL, # P<0.05, ## P<0.01).

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: Concentration Assay

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: HANPs could be endocytosed into 143B and UMR106 cells. Among the six HANPs, R-HANPs-1 had the highest internalization efficiency in 143B cells, and N-HANPs had the highest internalization efficiency in UMR106 cells. The representative flow cytometer histograms ( A and C ) and mean fluorescence intensity ( B and D ) of 143B (a, b) and UMR106 ( C and D ) cells after the cells treated with the six FITC-HANPs at the concentration of 400 μg/mL for 4 h (n=3; for 143B cells: vs control, **P<0.01; vs R-HANPs-1, $$ P<0.01; for UMR106 cells: vs control, **P<0.01; vs N-HANPs-1, ++ P<0.01; vs N-HANPs-2, && P<0.01).

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: Flow Cytometry, Fluorescence, Concentration Assay, Control

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: HANPs increased the level of intracellular Ca 2+ , depolarized mitochondria membrane potential and promoted ROS production of the tumor cells. The relative intracellular Ca 2+ fluorescence intensity of 143B ( A ) and UMR106 ( B ) cells after the cells were treated with six HANPs at the concentration of 400 μg/mL for 1, 2 and 3 days. The representative CLSM images of 143B and UMR106 cells by JC-1 staining after the cells were treated with six HANPs at the concentration of 400 μg/mL for 3 days (n=3; after HANPs treatment, the corresponding red fluorescence (JC-1 aggregates) decreased, while the corresponding green fluorescence (JC-1 monomers) increased, indicating that mitochondrial depolarization occurred in tumor cells after HANPs treatments) ( C ). The relative ROS fluorescence intensity in 143B ( D ) and UMR106 ( E ) cells after the cells were treated with six HANPs at the concentration of 400 μg/mL for 1, 2 and 3 days (n=3; for 143B cells: vs control, *P<0.05,**P<0.01; vs R-HANPs-1, $ P<0.05, $$ P<0.01; for UMR106 cells: vs control, *P<0.05,**P<0.01; vs N-HANPs-1, ++ P<0.01; vs N-HANPs-2, & P<0.05, && P<0.01).

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: Membrane, Fluorescence, Concentration Assay, Staining, Control

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: HANPs induced the apoptosis of 143B and UMR106 cells. The typical CLSM images of 143B and UMR106 cells stained with nuclei (blue) after the cells were treated with six HANPs at the concentration of 400 μg/mL for 3 days (n=3; white triangle: the nucleus shrinkage or fragmentation) ( A ). The apoptosis rates obtained by double-staining with Annexin V-FITC/PI of 143B ( B and C ) and UMR106 ( D and E ) cells after the cells treated with six HANPs at the concentration of 400 μg/mL for 1 ( B and D ) and 3 ( C and E ) days (n=3).

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: Staining, Concentration Assay, Double Staining

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: HANPs activated mitochondria apoptotic signaling in 143B and UMR106 cells. The gene expression level of mitochondria apoptosis related molecules in 143B ( A ) and UMR106 ( B ) cells after the cells were treated with six HANPs at the concentration of 400 μg/mL for 1, 2 and 3 days (n=3; for 143B cells: vs control, *P<0.05,**P<0.01; vs R-HANPs-1, $ P<0.05, $$ P<0.01; for UMR106 cells: vs control, *P<0.05,**P<0.01; vs N-HANPs-1, + P<0.05, ++ P<0.01; vs N-HANPs-2, & P<0.05, && P<0.01).

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: Gene Expression, Concentration Assay, Control

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: All the six HANPs inhibited the growth of 143B tumor tissues, while only N-HANPs inhibited the growth of UMR106 in the tumor-bearing mice model. Body weight ( A and F ) and tumor volume ( B and G ) in 143B ( A and B ) and UMR106 ( F and G ) OS-bearing mice after treated with six HANPs during the observation period. The typical images of tumors resected from 143B ( C ) and UMR106 ( H ) OS-bearing mice at day 28 and 21, respectively. The quantitative analysis of the resected tumor weight ( D and I ) and tumor volume ( E and J ) in 143B ( D and E ) and UMR106 ( I and J ) OS-bearing mice day 28 and 21, respectively (n=5; for 143B nude mice: vs control, *P<0.05, **P<0.01; vs R-HANPs-1, $ P<0.05; for UMR106 nude mice: vs control, *P<0.05, **P<0.01; vs N-HANPs-1, + P<0.05, ++ P<0.01; vs N-HANPs-2, & P<0.05, && P<0.01). H&E staining of the resected tumor tissue in 143B (k) and UMR106 (l) OS-bearing mice at day 28 and 21, respectively (scale bar: 250 μm).

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: Control, Staining

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: HANPs induced the apoptosis, inhibited the proliferation of OS tumor cells. Immunohistochemical staining and semi-quantification of TUNEL, Ki-67 and cyclin D1 of the resected 143B tumor tissues ( A-D ) and UMR106 tumor tissues ( E-H ) at day 28 and 21, respectively (for 143B nude mice: vs control, *P<0.05, **P<0.01; vs R-HANPs-1, $$ P<0.01; for UMR106 nude mice: vs control, *P<0.05, **P<0.01; vs N-HANPs-1, + P<0.05, ++ P<0.01; vs N-HANPs-2, && P<0.01, scale bar: 250 μm).

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: Immunohistochemical staining, Staining, TUNEL Assay, Control

Journal: International Journal of Nanomedicine

Article Title: A Selective Reduction of Osteosarcoma by Mitochondrial Apoptosis Using Hydroxyapatite Nanoparticles

doi: 10.2147/IJN.S375950

Figure Lengend Snippet: HANPs induced the apoptosis, inhibited the vascular differentiation and promoted CRT expression level in OS tumors. Immunohistochemical staining and semi-quantification of CD31, cytochrome C and CRT of the resected 143B tumor tissues ( A-D ) and UMR106 tumor tissues ( E-H ) at day 28 and 21, respectively (for 143B nude mice: vs control, *P<0.05, **P<0.01; vs R-HANPs-1, $$ P<0.01; for UMR106 nude mice: vs control, *P<0.05, **P<0.01; vs N-HANPs-1, ++ P<0.01; vs N-HANPs-2, & P<0.05, && P<0.01, scale bar: 250 μm).

Article Snippet: Human OS cell line (143B), rat OS cell line (UMR106) and mouse pre-osteoblast line (MC3T3-E1) were purchased from iCell Bioscience Inc (China).

Techniques: Expressing, Immunohistochemical staining, Staining, Control